sterile water Search Results


sterile distilled water  (Thermo Fisher)
95
Thermo Fisher sterile distilled water
Sterile Distilled Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Kanamycin%2C+in+distilled+water%2C+sterile-filtered/pmc11181181-81-16-22
Average 95 stars, based on 1 article reviews
sterile distilled water - by Bioz Stars, 2026-10
95/100 stars
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cells  (Thermo Fisher)
96
Thermo Fisher cells
Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Puromycin+dihydrochloride%2C+in+distilled+water%2C+sterile-filtered/pm42262924-783-0-8
Average 96 stars, based on 1 article reviews
cells - by Bioz Stars, 2026-10
96/100 stars
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fibroblast outgrowth  (Thermo Fisher)
94
Thermo Fisher fibroblast outgrowth
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Fibroblast Outgrowth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Amphotericin+B%2C+in+distilled+water%2C+sterile-filtered/pmc11339347-343-9-22
Average 94 stars, based on 1 article reviews
fibroblast outgrowth - by Bioz Stars, 2026-10
94/100 stars
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sterile water  (Bio-Rad)
94
Bio-Rad sterile water
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Sterile Water, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Sterile+Water/10__1128_slash_aem__71__11__7271___7278__2005-57-10-19
Average 94 stars, based on 1 article reviews
sterile water - by Bioz Stars, 2026-10
94/100 stars
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electroporation cuvettes  (Bio-Rad)
94
Bio-Rad electroporation cuvettes
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Sterile+Distilled+Water/pmc05429504-275-13-15
Average 94 stars, based on 1 article reviews
electroporation cuvettes - by Bioz Stars, 2026-10
94/100 stars
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distilled water  (Carolina Biological)
90
Carolina Biological distilled water
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Distilled Water, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Distilled+Water%2C+Sterile%2C+Jar/pmc05971843-408-27-8
Average 90 stars, based on 1 article reviews
distilled water - by Bioz Stars, 2026-10
90/100 stars
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ultrapure water  (Rockland Immunochemicals)
93
Rockland Immunochemicals ultrapure water
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Ultrapure Water, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Ultrapure+Sterile+Water/pmc07122782-117-24-26
Average 93 stars, based on 1 article reviews
ultrapure water - by Bioz Stars, 2026-10
93/100 stars
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neomycin sulfate  (Thermo Fisher)
94
Thermo Fisher neomycin sulfate
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Neomycin Sulfate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Neomycin+sulfate%2C+in+distilled+water%2C+sterile-filtered/pm38648490-372-0-2
Average 94 stars, based on 1 article reviews
neomycin sulfate - by Bioz Stars, 2026-10
94/100 stars
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sterile water  (Carolina Biological)
90
Carolina Biological sterile water
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Sterile Water, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Water%2C+Sterile/10__1155_slash_2016_slash_2547467-149-6-2
Average 90 stars, based on 1 article reviews
sterile water - by Bioz Stars, 2026-10
90/100 stars
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v v antibiotics penicillin streptomycin gibco invitrogen  (Thermo Fisher)
99
Thermo Fisher v v antibiotics penicillin streptomycin gibco invitrogen
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
V V Antibiotics Penicillin Streptomycin Gibco Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Streptomycin+sulfate%2C+in+distilled+water%2C+sterile-filtered/pmc12700216-220-17-20
Average 99 stars, based on 1 article reviews
v v antibiotics penicillin streptomycin gibco invitrogen - by Bioz Stars, 2026-10
99/100 stars
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v v gentamicin all gibco  (Thermo Fisher)
95
Thermo Fisher v v gentamicin all gibco
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
V V Gentamicin All Gibco, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Gentamicin+sulfate%2C+in+distilled+water%2C+sterile-filtered/10__1364_slash_oe__27__025749-217-23-26
Average 95 stars, based on 1 article reviews
v v gentamicin all gibco - by Bioz Stars, 2026-10
95/100 stars
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colisitin sulfate  (Thermo Fisher)
92
Thermo Fisher colisitin sulfate
(A) Western blot of <t>fibroblast</t> cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.
Colisitin Sulfate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sterile+water/Colistin+sulfate%2C+in+distilled+water%2C+sterile-filtered/pm39081042-305-32-30
Average 92 stars, based on 1 article reviews
colisitin sulfate - by Bioz Stars, 2026-10
92/100 stars
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Image Search Results


(A) Western blot of fibroblast cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.

Journal: Life Science Alliance

Article Title: CC2D1A causes ciliopathy, intellectual disability, heterotaxy, renal dysplasia, and abnormal CSF flow

doi: 10.26508/lsa.202402708

Figure Lengend Snippet: (A) Western blot of fibroblast cell line lysates from control, and Family#1 father, female patient, and male patient. The affected patient cell lines did not show detectable CC2D1A expression. (B, C) Quantification of fibroblast starvation–induced ciliary length. The patients displayed fewer ciliated cells or shorter ciliary lengths. ns, not significant; P < 0.01 (**), P < 0.0001 (****). (D, E, F) Cultured and starved fibroblasts from Family#1 father, female patient, and male patient stained with DAPI (blue) to show nuclei, phalloidin, Alexa Fluor 488 (green) to show actin cytoskeleton, and anti-acetylated tubulin (red) to show ciliary axonemes. Cilia on the patient fibroblasts were less frequent and shorter than those on the control and father fibroblasts. Scale bar = 25 μm. Source data are available for this figure.

Article Snippet: The fibroblasts were cultured for ∼4 wk until enough fibroblast outgrowth had occurred to allow for additional cell passage (amphotericin B [J67049.AD; Thermo Fisher Scientific], DMEM [#21068028; Gibco], L-glutamine [#25030081; Gibco], sodium pyruvate [#11360070; Gibco], penicillin–streptomycin [#10378016; Gibco], FBS [#10270-106; Gibco]).

Techniques: Western Blot, Control, Expressing, Cell Culture, Staining